Abstract: Biochemistry experiment teaching is an important auxiliary form of theoretical teaching. But there are many shortcomings in the teaching course at present.The combination of flipping classroom mode with reasonable part of traditional teaching mode can not only activate the atmosphere of experiment class, but also cultivate students’habit of independent learning and thinking,and improve students’problem-solving ability and innovative thinking ability. It researches and analyzes the application of comprehensive teaching model of“Flipping Classroom+Traditional Teaching”in biochemistry experiment teaching.