Abstract: In order to research the road performance of modified biological asphalt mixture, the water stability, the high temperature stability and the low temperature anti-crack resistance of different kinds of asphalt mixture were tested. The experiment results showed that the addition of modified asphalt could effectively prevent the erosion of water on the interface of asphalt and aggregate, and improve the water stability of asphalt mixture. Compared with the biological asphalt mixture, the freeze-thaw splitting strength ratio of modified biological asphalt mixture was significantly increased. The addition of modified asphalt could affect the high temperature performance of biological asphalt mixture and improve the original hard and brittle performance. At low temperature, the ultimate flexural strain and flexural tensile strength of the modified biological asphalt mixture could meet the requirements of technical index, and it had a low flexural stiffness modulus, showed a good low temperature anti-crack resistance.